SARS-CoV-Encoded Small RNAs Contribute to Infection-Associated Lung Pathology.

نویسندگان

  • Lucía Morales
  • Juan Carlos Oliveros
  • Raúl Fernandez-Delgado
  • Benjamin Robert tenOever
  • Luis Enjuanes
  • Isabel Sola
چکیده

Severe acute respiratory syndrome coronavirus (SARS-CoV) causes lethal disease in humans, which is characterized by exacerbated inflammatory response and extensive lung pathology. To address the relevance of small non-coding RNAs in SARS-CoV pathology, we deep sequenced RNAs from the lungs of infected mice and discovered three 18-22 nt small viral RNAs (svRNAs). The three svRNAs were derived from the nsp3 (svRNA-nsp3.1 and -nsp3.2) and N (svRNA-N) genomic regions of SARS-CoV. Biogenesis of CoV svRNAs was RNase III, cell type, and host species independent, but it was dependent on the extent of viral replication. Antagomir-mediated inhibition of svRNA-N significantly reduced in vivo lung pathology and pro-inflammatory cytokine expression. Taken together, these data indicate that svRNAs contribute to SARS-CoV pathogenesis and highlight the potential of svRNA-N antagomirs as antivirals.

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عنوان ژورنال:
  • Cell host & microbe

دوره 21 3  شماره 

صفحات  -

تاریخ انتشار 2017